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Home / ELISA Kit

Human Taste receptor type 1 member 2 (TAS1R2) ELISA Kit

SKU : BHE13702700

Taste receptor type 1 member 2 (TAS1R2) is a G protein-coupled receptor with seven trans-membrane domains and is a component of the heterodimeric amino acid taste receptor T1R2+3. TAS1R2 is formed as a dimer of the TAS1R2 and TAS1R3 proteins. Moreover, the TAS1R2 protein is not functional without formation of the 2+3 heterodimer. Another interesting quality of the TAS1R2 and TAS1R1 genes is their spontaneous activity in the absence of the extracellular domains and binding ligands. This may mean that the extracellular domain regulates function of the receptor by preventing spontaneous action as well as binding to activating ligands such as sucrose.

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Mfr.No.	KTE60483
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Taste receptor type 1 member 2 (TAS1R2) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS1R2.
Alternative Names	TAS1R2; GPR71; T1R2; TR2; G protein-coupled receptor 71
Detection Method	Colorimetric
Kit Components	Human Taste receptor type 1 member 2 microplate Human Taste receptor type 1 member 2 standard Human Taste receptor type 1 member 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Taste receptor type 1 member 2 (TAS1R2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TAS1R2. No significant cross-reactivity or interference between Human TAS1R2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Taste receptor type 1 member 2 (TAS1R2) is a G protein-coupled receptor with seven trans-membrane domains and is a component of the heterodimeric amino acid taste receptor T1R2+3. TAS1R2 is formed as a dimer of the TAS1R2 and TAS1R3 proteins. Moreover, the TAS1R2 protein is not functional without formation of the 2+3 heterodimer. Another interesting quality of the TAS1R2 and TAS1R1 genes is their spontaneous activity in the absence of the extracellular domains and binding ligands. This may mean that the extracellular domain regulates function of the receptor by preventing spontaneous action as well as binding to activating ligands such as sucrose.
Notes	This Human Taste receptor type 1 member 2 (TAS1R2) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS1R2 in samples. An antibody specific for TAS1R2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAS1R2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAS1R2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAS1R2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	80834

Applications	ELISA
Applications Notes	This Human Taste receptor type 1 member 2 (TAS1R2) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS1R2 in samples. An antibody specific for TAS1R2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAS1R2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAS1R2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAS1R2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702700	KTE60483	48 T	$339		Add to Cart
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