Human Tartrate Resistant Acid Phosphatase(TRAP)ELISA Kit

Human Tartrate Resistant Acid Phosphatase(TRAP)ELISA Kit

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Mfr.No.

CSB-E13415h

Detection Range

0.47 ng/mL-30 ng/mL

Sensitivity

0.12 ng/mL

Reactivity

Human

Sample Type

Serum, Tissue homogenates, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Tartrate Resistant Acid Phosphatase(TRAP)

Recovery

The recovery of human PD-L1/CD274 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-104
EDTA plasma (n=4)	95	90-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PD-L1/CD274 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	80-101
1:2	Average %	95
	Range %	91-105
1:4	Average %	98
	Range %	90-107
1:8	Average %	95
	Range %	85-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.274	2.174	2.224	1.965
500	1.940	1.854	1.897	1.638
250	1.450	1.355	1.403	1.144
125	0.927	0.955	0.941	0.682
62.5	0.570	0.580	0.575	0.316
31.2	0.422	0.417	0.420	0.161
15.6	0.352	0.341	0.347	0.088
0	0.253	0.265	0.259	?

Species

Homo sapiens

Limit Of Detection

450 nm

Antigen	Tartrate Resistant Acid Phosphatase(TRAP)

Applications

ELISA

Research Area

TRAP

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	The Circadian Gene Clock Regulates Bone Formation Via PDIA3 Yuan G.et al,J Bone Miner Res. ,Nov 24.

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SKU	Supplier No.	Size	Your price
BHE10504489	CSB-E13415h	96T	$790	Add to Cart
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BHE10504489	CSB-E13415h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.