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Home / ELISA Kit

Human Target of EGR1 protein 1 (TOE1) ELISA Kit

SKU : BHE13702388

Target of EGR1 protein 1 is a protein encoded by the TOE1 gene.Egr1, an immediate early transcription factor, responds to diverse stimuli and affects gene transcription to accomplish its biological effects. One important effect of Egr1 expression is to decrease the growth and tumorigenic potential of several tumor cell types. To identify important Egr1 target genes, adapted a methodology involving formaldehyde-induced protein-DNA cross-linking, chromatin immunoprecipitation, and multiplex PCR. Using this approach, report the cloning of a new Egr1 target gene that is able to account, at least in part, for the growth inhibitory activity of Egr1. TOE1 belongs to the CAF1 family. inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. mediates the inhibitory growth effect of EGR1.

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Mfr.No.	KTE60169
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Target of EGR1 protein 1 (TOE1) ELISA Kit employs a two-site sandwich ELISA to quantitate TOE1.
Alternative Names	TOE1; FLJ13949;
Detection Method	Colorimetric
Kit Components	Human Target of EGR1 protein 1 microplate Human Target of EGR1 protein 1 standard Human Target of EGR1 protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Target of EGR1 protein 1 (TOE1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TOE1. No significant cross-reactivity or interference between Human TOE1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Target of EGR1 protein 1 is a protein encoded by the TOE1 gene.Egr1, an immediate early transcription factor, responds to diverse stimuli and affects gene transcription to accomplish its biological effects. One important effect of Egr1 expression is to decrease the growth and tumorigenic potential of several tumor cell types. To identify important Egr1 target genes, adapted a methodology involving formaldehyde-induced protein-DNA cross-linking, chromatin immunoprecipitation, and multiplex PCR. Using this approach, report the cloning of a new Egr1 target gene that is able to account, at least in part, for the growth inhibitory activity of Egr1. TOE1 belongs to the CAF1 family. inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. mediates the inhibitory growth effect of EGR1.
Notes	This Human Target of EGR1 protein 1 (TOE1) ELISA Kit employs a two-site sandwich ELISA to quantitate TOE1 in samples. An antibody specific for TOE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	114034

Applications	ELISA
Applications Notes	This Human Target of EGR1 protein 1 (TOE1) ELISA Kit employs a two-site sandwich ELISA to quantitate TOE1 in samples. An antibody specific for TOE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702388	KTE60169	48 T	$339		Add to Cart
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BHE13702388	KTE60169	96 Tx50	$24569		Add to Cart

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