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Home / ELISA Kit

Human TAR DNA-binding protein 43 (TARDBP/TDP43) ELISA Kit

SKU : BHE13702695

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. TAR DNA-binding protein 43 (TARDBP/TDP43) is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, TARDBP/TDP43 regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.

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Mfr.No.	KTE60478
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human TAR DNA-binding protein 43 (TARDBP/TDP43) ELISA Kit employs a two-site sandwich ELISA to quantitate TARDBP.
Alternative Names	TARDBP; RP4-635E18.2; ALS10; TDP-43; TAR DNA-binding protein-43
Detection Method	Colorimetric
Kit Components	Human TAR DNA-binding protein 43 microplate Human TAR DNA-binding protein 43 standard Human TAR DNA-binding protein 43 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human TAR DNA-binding protein 43 (TARDBP/TDP43) ELISA Kit has high sensitivity and excellent specificity for detection of Human TARDBP. No significant cross-reactivity or interference between Human TARDBP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. TAR DNA-binding protein 43 (TARDBP/TDP43) is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, TARDBP/TDP43 regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
Notes	This Human TAR DNA-binding protein 43 (TARDBP/TDP43) ELISA Kit employs a two-site sandwich ELISA to quantitate TARDBP in samples. An antibody specific for TARDBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTARDBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TARDBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TARDBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	23435

Applications	ELISA
Applications Notes	This Human TAR DNA-binding protein 43 (TARDBP/TDP43) ELISA Kit employs a two-site sandwich ELISA to quantitate TARDBP in samples. An antibody specific for TARDBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTARDBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TARDBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TARDBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702695	KTE60478	48 T	$339		Add to Cart
BHE13702695	KTE60478	96 T	$589		Add to Cart
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BHE13702695	KTE60478	96 Tx50	$24569		Add to Cart

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