Human T-complex protein 1 subunit zeta(CCT6A) ELISA kit

Mfr.No.

CSB-EL004865HU

Alternative Names

chaperonin containing TCP1, subunit 6A (zeta 1), CCT-zeta, CCT-zeta-1, CCT6, Cctz, HTR3, MGC126214, MGC126215, MoDP-2, TCP-1-zeta, TCP20, TCPZ, TTCP20, T-complex protein 1, zeta subunit, acute morphine dependence related protein 2, amino acid transp

Reactivity

Human

Detection Range

25 pg/mL-1600 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse PAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	89	85-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	104
	Range %	101-109
1:2	Average %	95
	Range %	91-99
1:4	Average %	86
	Range %	83-89
1:8	Average %	92
	Range %	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.646	2.701	2.674	2.481
750	2.063	2.000	2.032	1.839
375	1.324	1.405	1.365	1.172
187.5	0.826	0.834	0.830	0.637
94	0.593	0.587	0.590	0.397
47	0.362	0.371	0.367	0.174
23.5	0.278	0.284	0.281	0.088
0	0.192	0.194	0.193	?

Species

Homo sapiens

Sensitivity

6.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	P40227
Antigen	chaperonin containing TCP1, subunit 6A (zeta 1)

Applications

ELISA

Research Area

CCT6A

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504511	CSB-EL004865HU	96T	$695	Add to Cart
BHE10504511	CSB-EL004865HU	5x96T	$3336	Add to Cart
BHE10504511	CSB-EL004865HU	10x96T	$6602.50	Add to Cart

Human T-complex protein 1 subunit zeta(CCT6A) ELISA kit

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