Human T-cell-specific Surface Glycoprotein Cd28

Mfr.No.

E4196hu

Description

This sandwich kit is for the accurate quantitative detection of human T-cell-specific Surface Glycoprotein CD28 (also known as CD28) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD 28;CD antigen CD28;CD28;T-cell-specific surface glycoprotein CD28;TP44

Reactivity

Human

Detection Range

0.05-30ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

25066

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.024ng/ml

Background

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed: 8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed: 15067037).

Gene ID	940
UniProt ID	P10747
Antigen	CD28

Research Area

Immunology

Protocol

These standard curves of E4196hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	2.098	2.214	2.158
	2.33
10ng/ml	1.695	1.595	1.539
	1.494
5ng/ml	1.112	1.078	1.022
	1.043
2.5ng/ml	0.712	0.712	0.656
	0.712
1.25ng/ml	0.426	0.447	0.392
	0.469
0ng/ml	0.061	0.056	0
	0.05

Precision

We measured random samples of E4196hu within the same batch/lot to ensure the consistency of the kits' performances.

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105596	E4196hu	48T	$320		Add to Cart
BHE12105596	E4196hu	96T	$458		Add to Cart

Human T-cell-specific Surface Glycoprotein Cd28 - CD28 ELISA Kit

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