Human T Cell Immunoreceptor with Ig and Itim Domains

Mfr.No.

E6245Hu

Alternative Names

T-cell immunoreceptor with Ig and ITIM domains;TIGIT;V-set and immunoglobulin domain-containing protein 9;V-set and transmembrane domain-containing protein 3;VSIG9;VSTM3;WUCAM

Reactivity

Human

Detection Range

0.05-20ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

26319

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.019ng/mL

Background

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Gene ID	201633
UniProt ID	Q495A1
Antigen	TIGIT

Research Area

Others

Protocol

These standard curves of E6245Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	2.287	2.275	2.229
	2.264
6ng/mL	1.632	1.564	1.518
	1.497
3ng/mL	1.142	1.124	1.077
	1.106
1.5ng/mL	0.716	0.745	0.698
	0.774
0.75ng/mL	0.467	0.447	0.4
	0.426
0ng/mL	0.039	0.047	0
	0.054

Precision

We measured random samples of E6245Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.56	0.12	7.6
Intra-Assay	2	18	5.62	0.25	4.39
Intra-Assay	3	18	2.37	0.2	8.29

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107607	E6245Hu	48T	$320		Add to Cart
BHE12107607	E6245Hu	96T	$458		Add to Cart

Human T Cell Immunoreceptor with Ig and Itim Domains - TIGIT ELISA Kit

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