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Home / ELISA Kit

Human Synaptotagmin-17 (SYT17) ELISA Kit

SKU : BHE13702649

Synaptotagmin-17 (SYT17) belongs to the synaptotagmin family, which is a group of membrane-trafficking proteins that contain two C-terminal C2 domains. Although synaptotagmins have been found to have a unique N-terminal domain that is involved in membrane anchoring (e.g., synaptotagmin) or specific ligand binding (e.g., rabphilin-3A and Doc2, IPR014638), SYT17 does not contain this domain.

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Mfr.No.	KTE60432
Description	This Human Synaptotagmin-17 (SYT17) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT17.
Alternative Names	SYT17; B/K protein
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Synaptotagmin-17 microplate Human Synaptotagmin-17 standard Human Synaptotagmin-17 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Synaptotagmin-17 (SYT17) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYT17. No significant cross-reactivity or interference between Human SYT17 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Synaptotagmin-17 (SYT17) belongs to the synaptotagmin family, which is a group of membrane-trafficking proteins that contain two C-terminal C2 domains. Although synaptotagmins have been found to have a unique N-terminal domain that is involved in membrane anchoring (e.g., synaptotagmin) or specific ligand binding (e.g., rabphilin-3A and Doc2, IPR014638), SYT17 does not contain this domain.
Notes	This Human Synaptotagmin-17 (SYT17) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT17 in samples. An antibody specific for SYT17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	51760
Antigen	SYT17
Tag	SYT17
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Synaptotagmin-17 (SYT17) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT17 in samples. An antibody specific for SYT17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702649	KTE60432	48 T	$339		Add to Cart
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BHE13702649	KTE60432	96 Tx50	$24569		Add to Cart

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