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Home / ELISA Kit

Human Synaptogyrin-4 (SYNGR4) ELISA Kit

SKU : BHE13702637

Synaptic vesicles represent specialized secretory organelles that store neurotransmitters in nerve terminals, and release them by fusing with the presynaptic plasma membrane during exocytosis. Synaptogyrin (SYNGR1) and synaptophysins I and II (synaptoporin) contain 4 transmembrane regions with cytoplasmic C and N termini, and are among the most abundant synaptic vesicle proteins.For a description of the synaptogyrin family, which includes SYNGR4.By genomic sequence analysis, Smith et al. (2000) identified SYNGR4 within a 1.6-Mb glioma tumor suppressor region on chromosome 19q. The SYNGR4 protein contains 234 amino acids.Smith et al. (2000) determined that the SYNGR4 gene contains 4 exons.By genomic sequence analysis, Smith et al. (2000) mapped the SYNGR4 gene to chromosome 19q13.3.

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Mfr.No.	KTE60420
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Synaptogyrin-4 (SYNGR4) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNGR4.
Alternative Names	SYNGR4; MGC125805;
Detection Method	Colorimetric
Kit Components	Human Synaptogyrin-4 microplate Human Synaptogyrin-4 standard Human Synaptogyrin-4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Synaptogyrin-4 (SYNGR4) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYNGR4. No significant cross-reactivity or interference between Human SYNGR4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Synaptic vesicles represent specialized secretory organelles that store neurotransmitters in nerve terminals, and release them by fusing with the presynaptic plasma membrane during exocytosis. Synaptogyrin (SYNGR1) and synaptophysins I and II (synaptoporin) contain 4 transmembrane regions with cytoplasmic C and N termini, and are among the most abundant synaptic vesicle proteins.For a description of the synaptogyrin family, which includes SYNGR4.By genomic sequence analysis, Smith et al. (2000) identified SYNGR4 within a 1.6-Mb glioma tumor suppressor region on chromosome 19q. The SYNGR4 protein contains 234 amino acids.Smith et al. (2000) determined that the SYNGR4 gene contains 4 exons.By genomic sequence analysis, Smith et al. (2000) mapped the SYNGR4 gene to chromosome 19q13.3.
Notes	This Human Synaptogyrin-4 (SYNGR4) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNGR4 in samples. An antibody specific for SYNGR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNGR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNGR4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNGR4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	23546

Applications	ELISA
Applications Notes	This Human Synaptogyrin-4 (SYNGR4) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNGR4 in samples. An antibody specific for SYNGR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNGR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNGR4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNGR4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702637	KTE60420	48 T	$339		Add to Cart
BHE13702637	KTE60420	96 T	$589		Add to Cart
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