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Home / ELISA Kit

Human Surfactant-associated protein 2 (SFTA2) ELISA Kit

SKU : BHE13702893

SFTPGis an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period.

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Mfr.No.	KTE60677
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Surfactant-associated protein 2 (SFTA2) ELISA Kit employs a two-site sandwich ELISA to quantitate SFTA2.
Alternative Names	SFTA2; DADB-160J14.6; GSGL541; SFTPG; UNQ541; surfactant associated protein G
Detection Method	Colorimetric
Kit Components	Human Surfactant-associated protein 2 microplate Human Surfactant-associated protein 2 standard Human Surfactant-associated protein 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Surfactant-associated protein 2 (SFTA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SFTA2. No significant cross-reactivity or interference between Human SFTA2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SFTPGis an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period.
Notes	This Human Surfactant-associated protein 2 (SFTA2) ELISA Kit employs a two-site sandwich ELISA to quantitate SFTA2 in samples. An antibody specific for SFTA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFTA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	389376

Applications	ELISA
Applications Notes	This Human Surfactant-associated protein 2 (SFTA2) ELISA Kit employs a two-site sandwich ELISA to quantitate SFTA2 in samples. An antibody specific for SFTA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFTA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702893	KTE60677	48 T	$339		Add to Cart
BHE13702893	KTE60677	96 T	$589		Add to Cart
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BHE13702893	KTE60677	96 Tx50	$24569		Add to Cart

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