Human Superoxide dismutase [Mn], mitochondrial (SOD2) ELISA kit

Human Superoxide dismutase [Mn], mitochondrial (SOD2) ELISA kit

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Mfr.No.

CSB-E17064h

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

superoxide dismutase 2, mitochondrial, IPO-B, MNSOD, MVCD6, Mn-SOD, Mn superoxide dismutase, indophenoloxidase B, manganese-containing superoxide dismutase, mangano-superoxide dismutase

Recovery

The recovery of rat IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-98
EDTA plasma (n=4)	93	89-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	101
	Range %	97-104
1:2000	Average %	92
	Range %	88-96
1:4000	Average %	90
	Range %	86-94
1:8000	Average %	103
	Range %	99-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
2000	0.238	0.231	0.235
500	0.447	0.424	0.436
125	0.692	0.657	0.675
31.25	1.224	1.285	1.255
7.81	1.798	1.737	1.768
0	2.063	2.045	2.054

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P04179

Applications	ELISA
Research Area	SOD2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504463	CSB-E17064h	96T	$695	Add to Cart
BHE10504463	CSB-E17064h	5x96T	$3336	Add to Cart
BHE10504463	CSB-E17064h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.