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Home / ELISA Kit

Human SUMO-conjugating enzyme UBC9 (UBE2I/UBC9/UBCE9) ELISA Kit

SKU : BHE13702320

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s.
UBE2I encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.The protein has a predicted sequence of 158 amino acids and can support the growth of yeast ubc9 temperature-sensitive mutants at non-permissive temperatures, indicating that their gene is a functional homolog of yeast ubc9.

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Mfr.No.	KTE60101
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human SUMO-conjugating enzyme UBC9 (UBE2I/UBC9/UBCE9) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2I.
Alternative Names	UBE2I; C358B7.1; P18; UBC9; OTTHUMP00000198126; OTTHUMP00000198127; SUMO-1-protein ligase; ubiquitin carrier protein; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme E2I; ubiquitin-conjugating enzym
Detection Method	Colorimetric
Kit Components	Human SUMO-conjugating enzyme UBC9 microplate Human SUMO-conjugating enzyme UBC9 standard Human SUMO-conjugating enzyme UBC9 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human SUMO-conjugating enzyme UBC9 (UBE2I/UBC9/UBCE9) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBE2I. No significant cross-reactivity or interference between Human UBE2I and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2I encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.The protein has a predicted sequence of 158 amino acids and can support the growth of yeast ubc9 temperature-sensitive mutants at non-permissive temperatures, indicating that their gene is a functional homolog of yeast ubc9.
Notes	This Human SUMO-conjugating enzyme UBC9 (UBE2I/UBC9/UBCE9) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2I in samples. An antibody specific for UBE2I has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE2I present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2I is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2I bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7329

Applications	ELISA
Applications Notes	This Human SUMO-conjugating enzyme UBC9 (UBE2I/UBC9/UBCE9) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2I in samples. An antibody specific for UBE2I has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE2I present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2I is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2I bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702320	KTE60101	48 T	$359		Add to Cart
BHE13702320	KTE60101	96 T	$619		Add to Cart
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BHE13702320	KTE60101	96 Tx50	$26209		Add to Cart

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