Human Sulfotransferase 1A1(SULT1A1) ELISA kit

Mfr.No.

CSB-EL022933HU

Alternative Names

sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1, HAST1/HAST2, MGC131921, MGC5163, P-PST, PST, ST1A3, STP, STP1, TSPST1, aryl sulfotransferase, phenol-preferring phenol sulfotransferase1, phenol-sulfating phenol sulfotransferase, thermostable phenol s

Reactivity

Human

Detection Range

25 pg/mL-1600 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse HTRA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-110
EDTA plasma (n=4)	93	89-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HTRA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-93
1:2	Average %	95
	Range %	90-100
1:4	Average %	91
	Range %	86-95
1:8	Average %	97
	Range %	92-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.635	2.492	2.564	2.374
2500	2.001	1.903	1.952	1.762
1250	1.329	1.357	1.343	1.153
625	0.814	0.860	0.837	0.647
312	0.547	0.519	0.533	0.343
156	0.352	0.338	0.345	0.155
78	0.263	0.271	0.267	0.077
0	0.186	0.194	0.190	?

Species

Homo sapiens

Sensitivity

6.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	P50225
Antigen	sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1

Applications

ELISA

Research Area

SULT1A1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504460	CSB-EL022933HU	96T	$695	Add to Cart
BHE10504460	CSB-EL022933HU	5x96T	$3336	Add to Cart
BHE10504460	CSB-EL022933HU	10x96T	$6602.50	Add to Cart

Human Sulfotransferase 1A1(SULT1A1) ELISA kit

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