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Home / ELISA Kit

Human Sulfite oxidase, mitochondrial (SUOX) ELISA Kit

SKU : BHE13702623

Sulfite oxidase (EC 1.8.3.1) is an enzyme in the mitochondria of all eukaryotes. It oxidizes sulfite to sulfate and, via cytochrome c, transfers the electrons produced to the electron transport chain, allowing generation of ATP in oxidative phosphorylation. This is the last step in the metabolism of sulfur-containing compounds and the sulfate is excreted.
Sulfite oxidase is a metallo-enzyme that utilizes a molybdopterin cofactor and a heme group. It is one of the cytochromes b5 and belongs to the enzyme super-family of oxotransferases that also includes DMSO reductase, xanthine oxidase, and nitrite reductase.In mammals, the expression levels of sulfite oxidase is high in the liver, kidney, and heart, and very low in spleen, brain, skeletal muscle, and blood.

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Mfr.No.	KTE60406
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Sulfite oxidase, mitochondrial (SUOX) ELISA Kit employs a two-site sandwich ELISA to quantitate SUOX.
Alternative Names	SUOX
Detection Method	Colorimetric
Kit Components	Human Sulfite oxidase, mitochondrial microplate Human Sulfite oxidase, mitochondrial standard Human Sulfite oxidase, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Sulfite oxidase, mitochondrial (SUOX) ELISA Kit has high sensitivity and excellent specificity for detection of Human SUOX. No significant cross-reactivity or interference between Human SUOX and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Sulfite oxidase (EC 1.8.3.1) is an enzyme in the mitochondria of all eukaryotes. It oxidizes sulfite to sulfate and, via cytochrome c, transfers the electrons produced to the electron transport chain, allowing generation of ATP in oxidative phosphorylation. This is the last step in the metabolism of sulfur-containing compounds and the sulfate is excreted. Sulfite oxidase is a metallo-enzyme that utilizes a molybdopterin cofactor and a heme group. It is one of the cytochromes b5 and belongs to the enzyme super-family of oxotransferases that also includes DMSO reductase, xanthine oxidase, and nitrite reductase.In mammals, the expression levels of sulfite oxidase is high in the liver, kidney, and heart, and very low in spleen, brain, skeletal muscle, and blood.
Notes	This Human Sulfite oxidase, mitochondrial (SUOX) ELISA Kit employs a two-site sandwich ELISA to quantitate SUOX in samples. An antibody specific for SUOX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySUOX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SUOX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SUOX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6821

Applications	ELISA
Applications Notes	This Human Sulfite oxidase, mitochondrial (SUOX) ELISA Kit employs a two-site sandwich ELISA to quantitate SUOX in samples. An antibody specific for SUOX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySUOX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SUOX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SUOX bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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