Human Sulfhydryloxidase 1 - QSOX1 ELISA Kit

Mfr.No.

E3450Hu

Description

This sandwich kit is for the accurate quantitative detection of human Sulfhydryl Oxidase 1 (also known as QSOX1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

hQSOX;QSOX 1;QSOX1;Quiescin Q6;Sulfhydryl oxidase 1

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

82578

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.15ng/L

Background

Catalyzes the oxidation of sulfhydryl groups in Peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide (PubMed: 17331072, PubMed: 18393449, PubMed: 23704371, PubMed: 30367560, PubMed: 23867277). Plays a role in disulfide bond formation in a variety of extracellular proteins (PubMed: 17331072, PubMed: 30367560, PubMed: 22801504, PubMed: 23867277). In fibroblasts, required for normal incorporation of laminin into the extracellular matrix, and thereby for normal cell-cell adhesion and cell migration (PubMed: 23704371, PubMed: 30367560, PubMed: 23867277).

Gene ID	5768
UniProt ID	O00391
Antigen	QSOX1

Research Area

Cell Biology

Protocol

These standard curves of E3450Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.035	2.052	1.997
	2.069
600ng/L	0.907	0.943	0.888
	0.978
300ng/L	0.477	0.467	0.412
	0.457
150ng/L	0.21	0.249	0.194
	0.288
75ng/L	0.113	0.104	0.05
	0.096
0ng/L	0.061	0.055	0
	0.049

Precision

We measured random samples of E3450Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1058.5	29.39	2.8
Intra-Assay	2	18	293.6	14.39	4.9
Intra-Assay	3	18	611.9	23.66	3.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104970	E3450Hu	48T	$320		Add to Cart
BHE12104970	E3450Hu	96T	$458		Add to Cart

Human Sulfhydryloxidase 1 - QSOX1 ELISA Kit

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