Human Stromelysin-1 - ST1 ELISA K

Mfr.No.

E1722Hu

Sensitivity

0.11ng/ml

Reactivity

Human

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Stromelysin-1 (also known as ST1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Matrix Metalloproteinase-3;MMP 3;MMP3;MMP-3;SL-1;Stromelysin-1;Transin-1

Molecular Weight

53977

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1722Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	25.7	0.78	3
Intra-Assay	2	18	5.7	0.25	4.4
Intra-Assay	3	18	18.4	0.89	4.8

Background

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Protocol

These standard curves of E1722Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.282	2.264	2.244
	2.245
20ng/ml	1.325	1.259	1.239
	1.193
10ng/ml	0.587	0.631	0.612
	0.676
5ng/ml	0.399	0.369	0.349
	0.339
2.5ng/ml	0.216	0.172	0.153
	0.129
0ng/ml	0.019	0.02	0
	0.02

Gene ID	4314
UniProt ID	P08254
Antigen	MMP3

Research Area	Cardiovascular; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103304	E1722Hu	48T	$320		Add to Cart
BHE12103304	E1722Hu	96T	$458		Add to Cart

Human Stromelysin-1 - ST1 ELISA K

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