Human Stress-associated Endoplasmic Reticulum Protein 1

Mfr.No.

E3316Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Adipose Triglyceride Lipase (also known as ATGL) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

RAMP4;Ribosome-attached membrane protein 4;SERP1;Stress-associated endoplasmic reticulum protein 1

Reactivity

Human

Detection Range

1-400ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

7374

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.57ng/ml

Background

Interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation during ER stress. May facilitate glycosylation of target proteins after termination of ER stress. May modulate the use of N-glycosylation sites on target proteins (By similarity).

Gene ID	27230
UniProt ID	Q9Y6X1
Antigen	SERP1

Research Area

Others

Protocol

These standard curves of E3316Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/ml	2.114	2.104	2.055
	2.095
120ng/ml	1.574	1.592	1.542
	1.61
60ng/ml	1.091	1.124	1.075
	1.157
30ng/ml	0.766	0.696	0.647
	0.627
15ng/ml	0.477	0.479	0.429
	0.481
0ng/ml	0.039	0.05	0
	0.06

Precision

We measured random samples of E3316Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	52.1	4.39	8.42
Intra-Assay	2	18	119.63	7.34	6.13
Intra-Assay	3	18	27.88	2.02	7.23

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104845	E3316Hu	48T	$320		Add to Cart
BHE12104845	E3316Hu	96T	$458		Add to Cart

Human Stress-associated Endoplasmic Reticulum Protein 1 - SERP1 ELISA Kit

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