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Home / ELISA Kit

Human Steroidogenic acute regulatory protein, mitochondrial (STAR) ELISA Kit

SKU : BHE13702591

The steroidogenic acute regulatory protein is a transport protein that regulates cholesterol transfer within the mitochondria, which is the rate-limiting step in the production of steroid hormones. It is primarily present in steroid-producing cells, including theca cells and luteal cells in the ovary, Leydig cells in the testis and cell types in the adrenal cortex. The domain of StAR important for promoting cholesterol transfer is the StAR-related transfer domain (START domain). StAR is the prototypic member of the START domain family of proteins and is thus also known as STARD1 for "START domain-containing protein 1". It is hypothesized that the START domain forms a pocket in StAR that binds single cholesterol molecules for delivery to P450scc.

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Mfr.No.	KTE60374
Description	This Human Steroidogenic acute regulatory protein, mitochondrial (STAR) ELISA Kit employs a two-site sandwich ELISA to quantitate STAR.
Alternative Names	STAR; STARD1; START domain containing 1; StAR-related lipid transfer (START) domain containing 1; cholesterol trafficker; mitochondrial steroid acute regulatory protein; steroid acute regulatory protein; stero
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Steroidogenic acute regulatory protein, mitochondrial microplate Human Steroidogenic acute regulatory protein, mitochondrial standard Human Steroidogenic acute regulatory protein, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Steroidogenic acute regulatory protein, mitochondrial (STAR) ELISA Kit has high sensitivity and excellent specificity for detection of Human STAR. No significant cross-reactivity or interference between Human STAR and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The steroidogenic acute regulatory protein is a transport protein that regulates cholesterol transfer within the mitochondria, which is the rate-limiting step in the production of steroid hormones. It is primarily present in steroid-producing cells, including theca cells and luteal cells in the ovary, Leydig cells in the testis and cell types in the adrenal cortex. The domain of StAR important for promoting cholesterol transfer is the StAR-related transfer domain (START domain). StAR is the prototypic member of the START domain family of proteins and is thus also known as STARD1 for "START domain-containing protein 1". It is hypothesized that the START domain forms a pocket in StAR that binds single cholesterol molecules for delivery to P450scc.
Notes	This Human Steroidogenic acute regulatory protein, mitochondrial (STAR) ELISA Kit employs a two-site sandwich ELISA to quantitate STAR in samples. An antibody specific for STAR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTAR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STAR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STAR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	6770
Antigen	STAR
Tag	STAR
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Steroidogenic acute regulatory protein, mitochondrial (STAR) ELISA Kit employs a two-site sandwich ELISA to quantitate STAR in samples. An antibody specific for STAR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTAR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STAR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STAR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702591	KTE60374	48 T	$339		Add to Cart
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