Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine®

Mfr.No.

EK1988

Sensitivity

<5 pg/ml

Detection Range

7.8 pg/ml - 500 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	7.8	15.6	31.2	62.5	125	250	500
O.D.	0.033	0.082	0.135	0.214	0.402	0.731	1.288	2.062

Description

Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine® (96 Tests). Quantitate Human STC2 in cell culture supeatants and serum. Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Stanniocalcin-2; STC-2; Stanniocalcin-related protein; STC-related protein; STCRP; STC2

Immunogen

Expression system for standard: NS0; Immunogen sequence: T25-R302

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human STC2

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	9	71	207	12	75	219
Standard deviation	0.38	3.62	11.39	0.82	4.43	13.36
CV (%)	4.2%	5.1%	5.5%	6.8%	5.9%	6.1%

Protein Function

Has an anti-hypocalcemic action on calcium and phosphate homeostasis.

Background

Stanniocalcin-2 is a protein that in humans is encoded by the STC2 gene. It is mapped to 5q35.2. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	9	10	9	10	9	0.50	5.5%
Sample 2	71	72	61	64	67	4.63	6.9%
Sample 3	207	217	226	208	214	7.69	3.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	8614
Gene Name	STC2
UniProt ID	O76061
Tissue Specificity	Expressed in a variety of tissues including muscle, heart, pancreas, kidney, spleen, prostate, small intestine, colon and peripheral blood leukocytes.

Applications	ELISA
Research Area	Calcium Signaling,Mesenchymal Stem Cells,Secreted Molecules,Signal Transduction,Signaling Pathway,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001493	EK1988	96 wells/kit	$750		Add to Cart

Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine®

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