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Home / ELISA Kit

Human Stathmin-1 (STMN1) ELISA Kit

SKU : BHE13702608

Stathmin 1/oncoprotein 18, also known as STMN1, is a highly conserved 17 kDa protein. Its function as an important regulatory protein of microtubule dynamics has been well characterized. Eukaryotic microtubules are one of three major components of the cell s cytoskeleton. They are highly dynamic structures that continuously alternate between assembly and disassembly. Stathmin performs an important function in regulating rapid microtubule remodeling of the cytoskeleton in response to the cell s needs. Microtubules are cylindrical polymers of alpha,beta-tubulin. Their assembly is in part determined by the concentration of free tubulin in the cytoplasm.At low concentrations of free tubulin, the growth rate at the microtubule ends is slowed and results in an increased rate of depolymerization (disassembly).

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Mfr.No.	KTE60391
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Stathmin-1 (STMN1) ELISA Kit employs a two-site sandwich ELISA to quantitate STMN1.
Alternative Names	STMN1; RP1-125I3.5; C1orf215; FLJ32206; LAP18; Lag; MGC138869; MGC138870; OP18; PP17; PP19; PR22; SMN; OTTHUMP00000008530; leukemia-associated phosphoprotein p18; metablastin; oncoprotein 18; phosphoprotein 19
Detection Method	Colorimetric
Kit Components	Human Stathmin-1 microplate Human Stathmin-1 standard Human Stathmin-1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Stathmin-1 (STMN1) ELISA Kit has high sensitivity and excellent specificity for detection of Human STMN1. No significant cross-reactivity or interference between Human STMN1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Stathmin 1/oncoprotein 18, also known as STMN1, is a highly conserved 17 kDa protein. Its function as an important regulatory protein of microtubule dynamics has been well characterized. Eukaryotic microtubules are one of three major components of the cell s cytoskeleton. They are highly dynamic structures that continuously alternate between assembly and disassembly. Stathmin performs an important function in regulating rapid microtubule remodeling of the cytoskeleton in response to the cell s needs. Microtubules are cylindrical polymers of alpha,beta-tubulin. Their assembly is in part determined by the concentration of free tubulin in the cytoplasm.At low concentrations of free tubulin, the growth rate at the microtubule ends is slowed and results in an increased rate of depolymerization (disassembly).
Notes	This Human Stathmin-1 (STMN1) ELISA Kit employs a two-site sandwich ELISA to quantitate STMN1 in samples. An antibody specific for STMN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTMN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STMN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STMN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	3925

Applications	ELISA
Applications Notes	This Human Stathmin-1 (STMN1) ELISA Kit employs a two-site sandwich ELISA to quantitate STMN1 in samples. An antibody specific for STMN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTMN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STMN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STMN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702608	KTE60391	48 T	$359		Add to Cart
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