Matrices listed below were spiked with certain level of STAT6 and the recovery rates were calculated by comparing the measured value to the expected amount of STAT6 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
82-101
93
EDTA plasma(n=5)
84-105
93
heparin plasma(n=5)
80-104
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of STAT6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
81-105%
86-104%
87-98%
EDTA plasma(n=5)
94-98%
90-98%
94-101%
heparin plasma(n=5)
92-94%
91-100%
97-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
P42226
Applications
ELISA
Applications Notes
STAT6 ELISA Kit allows for the in vitro quantitative determination of STAT6 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human STAT6(Signal transducer and activator of transcription 6) ELISA Kit