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Home / ELISA Kit

Human Stanniocalcin-1 (STC1) ELISA Kit

SKU : BHE13702600

Stanniocalcin-1 is a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The gene contains a 5' UTR rich in CAG trinucleotide repeats. The encoded protein contains 11 conserved cysteine residues and is phosphorylated by protein kinase C exclusively on its serine residues.
The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Overexpression of human stanniocalcin 1 in mice produces high serum phosphate levels, dwarfism, and increased metabolic rate. This gene has altered expression in hepatocellular, ovarian, and breast cancers.

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Mfr.No.	KTE60383
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Stanniocalcin-1 (STC1) ELISA Kit employs a two-site sandwich ELISA to quantitate STC1.
Alternative Names	STC1; STC;
Detection Method	Colorimetric
Kit Components	Human Stanniocalcin-1 microplate Human Stanniocalcin-1 standard Human Stanniocalcin-1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Stanniocalcin-1 (STC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human STC1. No significant cross-reactivity or interference between Human STC1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Stanniocalcin-1 is a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The gene contains a 5' UTR rich in CAG trinucleotide repeats. The encoded protein contains 11 conserved cysteine residues and is phosphorylated by protein kinase C exclusively on its serine residues. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Overexpression of human stanniocalcin 1 in mice produces high serum phosphate levels, dwarfism, and increased metabolic rate. This gene has altered expression in hepatocellular, ovarian, and breast cancers.
Notes	This Human Stanniocalcin-1 (STC1) ELISA Kit employs a two-site sandwich ELISA to quantitate STC1 in samples. An antibody specific for STC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6781

Applications	ELISA
Applications Notes	This Human Stanniocalcin-1 (STC1) ELISA Kit employs a two-site sandwich ELISA to quantitate STC1 in samples. An antibody specific for STC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702600	KTE60383	48 T	$339		Add to Cart
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BHE13702600	KTE60383	96 Tx50	$24569		Add to Cart

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