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Home / ELISA Kit

Human Squalene monooxygenase (SQLE) ELISA Kit

SKU : BHE13702570

Squalene epoxidase (EC 1.14.99.7) catalyzes the first oxygenation step in sterol biosynthesis and is thought to be one of the rate-limiting enzymes in this pathway. Squalene epoxidase (SE) is a key flavin adenine dinucleotide (FAD)-dependent enzyme of ergosterol and cholesterol biosynthetic pathways and an attractive potential target for drugs used to inhibit the growth of pathogenic fungi or to lower cholesterol level. By PCR analysis of a human/rodent somatic cell hybrid mapping panel, Nagai et al. (1997) demonstrated that the human SQLE gene maps to chromosome 8. The localization was refined by PCR analysis of a radiation hybrid panel. The results showed that human SQLE is most tightly linked to D8S508, which is reported to be located at 8q24.13-qter. The authors used fluorescence in situ hybridization to map SQLE to 8q24.1.

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Mfr.No.	KTE60353
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Squalene monooxygenase (SQLE) ELISA Kit employs a two-site sandwich ELISA to quantitate SQLE.
Alternative Names	SQLE; FLJ30795; squalene monooxygenase
Detection Method	Colorimetric
Kit Components	Human Squalene monooxygenase microplate Human Squalene monooxygenase standard Human Squalene monooxygenase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Squalene monooxygenase (SQLE) ELISA Kit has high sensitivity and excellent specificity for detection of Human SQLE. No significant cross-reactivity or interference between Human SQLE and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Squalene epoxidase (EC 1.14.99.7) catalyzes the first oxygenation step in sterol biosynthesis and is thought to be one of the rate-limiting enzymes in this pathway. Squalene epoxidase (SE) is a key flavin adenine dinucleotide (FAD)-dependent enzyme of ergosterol and cholesterol biosynthetic pathways and an attractive potential target for drugs used to inhibit the growth of pathogenic fungi or to lower cholesterol level. By PCR analysis of a human/rodent somatic cell hybrid mapping panel, Nagai et al. (1997) demonstrated that the human SQLE gene maps to chromosome 8. The localization was refined by PCR analysis of a radiation hybrid panel. The results showed that human SQLE is most tightly linked to D8S508, which is reported to be located at 8q24.13-qter. The authors used fluorescence in situ hybridization to map SQLE to 8q24.1.
Notes	This Human Squalene monooxygenase (SQLE) ELISA Kit employs a two-site sandwich ELISA to quantitate SQLE in samples. An antibody specific for SQLE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySQLE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SQLE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SQLE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6713

Applications	ELISA
Applications Notes	This Human Squalene monooxygenase (SQLE) ELISA Kit employs a two-site sandwich ELISA to quantitate SQLE in samples. An antibody specific for SQLE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySQLE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SQLE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SQLE bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702570	KTE60353	48 T	$339		Add to Cart
BHE13702570	KTE60353	96 T	$589		Add to Cart
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BHE13702570	KTE60353	96 Tx50	$24569		Add to Cart

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