Matrices listed below were spiked with certain level of SPTBN2 and the recovery rates were calculated by comparing the measured value to the expected amount of SPTBN2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
94-102
99
EDTA plasma(n=5)
87-102
96
heparin plasma(n=5)
91-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPTBN2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-102%
87-97%
87-105%
EDTA plasma(n=5)
88-94%
83-100%
86-97%
heparin plasma(n=5)
87-100%
81-96%
83-89%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
O15020
Applications
ELISA
Applications Notes
SPTBN2 ELISA Kit allows for the in vitro quantitative determination of SPTBN2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human SPTBN2(Spectrin beta chain, non-erythrocytic 2) ELISA Kit