Human Spry Domain-containing Socs Box Protein 3

Mfr.No.

E3180Hu

Alternative Names

C16orf31;SPRY domain-containing SOCS box protein 3;SPSB3;SSB3;SSB-3

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

39376

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.14ng/L

Background

May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Gene ID	90864
UniProt ID	Q6PJ21
Antigen	SPSB3

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E3180Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.34	2.253	2.204
	2.167
800ng/L	1.144	1.231	1.182
	1.318
400ng/L	0.807	0.776	0.727
	0.746
200ng/L	0.432	0.462	0.413
	0.493
100ng/L	0.2	0.212	0.163
	0.224
0ng/L	0.051	0.049	0
	0.048

Precision

We measured random samples of E3180Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	594.9	34.89	5.86
Intra-Assay	2	18	1202.42	35.69	2.97
Intra-Assay	3	18	244.65	13.75	5.62

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104709	E3180Hu	48T	$320		Add to Cart
BHE12104709	E3180Hu	96T	$458		Add to Cart

Human Spry Domain-containing Socs Box Protein 3 - SPSB3 ELISA Kit

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