Human Spry Domain-containing Socs Box Protein 2

Mfr.No.

E3179Hu

Sensitivity

5.12ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Gene-rich cluster protein C9;GRCC9;SPRY domain-containing SOCS box protein 2;SPSB2;SSB2;SSB-2

Molecular Weight

28630

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E3179Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1050.29	33.81	3.22
Intra-Assay	2	18	733.8	32.99	4.5
Intra-Assay	3	18	279.54	13.22	4.73

Background

Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:15601820, PubMed:21199876). Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (PubMed:21199876). Acts as a bridge which links NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (PubMed:21199876).

Protocol

These standard curves of E3179Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	1.681	1.847	1.79
	2.013
600ng/L	1.055	1.027	0.97
	1
300ng/L	0.593	0.514	0.457
	0.434
150ng/L	0.249	0.214	0.157
	0.18
75ng/L	0.067	0.102	0.044
	0.136
0ng/L	0.052	0.057	0
	0.063

Gene ID	84727
UniProt ID	Q99619

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104708	E3179Hu	48T	$320		Add to Cart
BHE12104708	E3179Hu	96T	$458		Add to Cart

Human Spry Domain-containing Socs Box Protein 2 - SPSB2 ELISA Kit

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