Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of SPR and the recovery rates were calculated by comparing the measured value to the expected amount of SPR in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-97
92
EDTA plasma(n=5)
88-105
92
heparin plasma(n=5)
88-102
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-102%
85-100%
85-99%
EDTA plasma(n=5)
82-95%
82-88%
87-101%
heparin plasma(n=5)
81-98%
85-96%
82-98%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
P35270
Applications
ELISA
Applications Notes
SPR ELISA Kit allows for the in vitro quantitative determination of SPR concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human SPR (Sepiapterin reductase) ELISA Kit