Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of SPHK2 and the recovery rates were calculated by comparing the measured value to the expected amount of SPHK2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-95
90
EDTA plasma(n=5)
87-101
94
heparin plasma(n=5)
86-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPHK2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-102%
96-102%
94-102%
EDTA plasma(n=5)
85-101%
86-94%
87-99%
heparin plasma(n=5)
86-98%
88-99%
85-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q9NRA0
Applications
ELISA
Applications Notes
SPHK2 ELISA Kit allows for the in vitro quantitative determination of SPHK2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human SPHK2 (Sphingosine kinase 2) ELISA Kit