Matrices listed below were spiked with certain level of SPHK1 and the recovery rates were calculated by comparing the measured value to the expected amount of SPHK1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
97
EDTA plasma(n=5)
86-102
94
heparin plasma(n=5)
87-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPHK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
92-98%
91-100%
89-100%
EDTA plasma(n=5)
87-100%
86-93%
86-94%
heparin plasma(n=5)
85-92%
87-97%
85-96%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q9NYA1
Applications
ELISA
Applications Notes
SPHK1 ELISA Kit allows for the in vitro quantitative determination of SPHK1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human SPHK1(Sphingosine Kinase 1) ELISA Kit