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Home / ELISA Kit

Human Sphingosine (SPH) ELISA Kit

SKU : BHE13702747

Sphingosine (2-amino-4-octadecene-1,3-diol) is an 18-carbon amino alcohol with an unsaturated hydrocarbon chain, which forms a primary part of sphingolipids, a class of cell membrane lipids that include sphingomyelin, an important phospholipid.

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTE60531
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Sphingosine (SPH) ELISA Kit employs a two-site sandwich ELISA to quantitate SPH.
Alternative Names	SPH
Detection Method	Colorimetric
Kit Components	Human Sphingosine microplate Human Sphingosine standard Human Sphingosine detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Sphingosine (SPH) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPH. No significant cross-reactivity or interference between Human SPH and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Sphingosine (2-amino-4-octadecene-1,3-diol) is an 18-carbon amino alcohol with an unsaturated hydrocarbon chain, which forms a primary part of sphingolipids, a class of cell membrane lipids that include sphingomyelin, an important phospholipid.
Notes	This Human Sphingosine (SPH) ELISA Kit employs a two-site sandwich ELISA to quantitate SPH in samples. An antibody specific for SPH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Sphingosine (SPH) ELISA Kit employs a two-site sandwich ELISA to quantitate SPH in samples. An antibody specific for SPH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702747	KTE60531	48 T	$359		Add to Cart
BHE13702747	KTE60531	96 T	$619		Add to Cart
BHE13702747	KTE60531	96 Tx5	$2809		Add to Cart
BHE13702747	KTE60531	96 Tx50	$26209		Add to Cart

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