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Home / ELISA Kit

Human Sphingosine 1-phosphate receptor type 1 (S1P1) ELISA Kit

SKU : BHE13702972

S1PR1 (sphingosine-1-phosphate receptor 1) also known as S1P1 and EDG1 (endothelial differentiation gene 1) is a protein encoded by the S1PR1 gene. S1PR1 encodes a G-protein-coupled receptor which binds the lipid signaling molecule sphingosine 1-phosphate (S1P).
The protein is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion.Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.

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Mfr.No.	KTE60756
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Sphingosine 1-phosphate receptor type 1 (S1P1) ELISA Kit employs a two-site sandwich ELISA to quantitate S1PR1.
Alternative Names	S1PR1; CHEDG1; D1S3362; ECGF1; EDG-1; EDG1; FLJ58121; S1P1; endothelial differentiation; sphingolipid G-protein-coupled receptor; 1; sphingosine 1-phosphate receptor EDG1
Detection Method	Colorimetric
Kit Components	Human Sphingosine 1-phosphate receptor type 1 microplate Human Sphingosine 1-phosphate receptor type 1 standard Human Sphingosine 1-phosphate receptor type 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Sphingosine 1-phosphate receptor type 1 (S1P1) ELISA Kit has high sensitivity and excellent specificity for detection of Human S1PR1. No significant cross-reactivity or interference between Human S1PR1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	S1PR1 (sphingosine-1-phosphate receptor 1) also known as S1P1 and EDG1 (endothelial differentiation gene 1) is a protein encoded by the S1PR1 gene. S1PR1 encodes a G-protein-coupled receptor which binds the lipid signaling molecule sphingosine 1-phosphate (S1P). The protein is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion.Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
Notes	This Human Sphingosine 1-phosphate receptor type 1 (S1P1) ELISA Kit employs a two-site sandwich ELISA to quantitate S1PR1 in samples. An antibody specific for S1PR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS1PR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S1PR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S1PR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	1901

Applications	ELISA
Applications Notes	This Human Sphingosine 1-phosphate receptor type 1 (S1P1) ELISA Kit employs a two-site sandwich ELISA to quantitate S1PR1 in samples. An antibody specific for S1PR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS1PR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S1PR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S1PR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702972	KTE60756	48 T	$339		Add to Cart
BHE13702972	KTE60756	96 T	$589		Add to Cart
BHE13702972	KTE60756	96 Tx5	$2629		Add to Cart
BHE13702972	KTE60756	96 Tx50	$24569		Add to Cart

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