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Home / ELISA Kit

Human Spermine oxidase (SMOX) ELISA Kit

SKU : BHE13702784

Spermine oxidase is the polyamine oxidase. This enzyme potentially represents a new class of catabolic enzymes in the mammalian polyamine metabolic pathway capable of the efficient oxidation of polyamines. More than five transcript variants encoding four active isoenzymes have been identified for this gene, however, not all variants have been fully described. The characterized isoenzymes have distinctive biochemical characteristics and substrate specificities, suggesting the existence of additional levels of complexity in polyamine catabolism.Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal.

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Mfr.No.	KTE60568
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Spermine oxidase (SMOX) ELISA Kit employs a two-site sandwich ELISA to quantitate SMOX.
Alternative Names	SMOX; C20orf16; FLJ20746; MGC1010; PAO; PAOH1; SMO; OTTHUMP00000030158; flavin containing amine oxidase; flavin-containing spermine oxidase; polyamine oxidase; putative cyclin G1 interacting protein
Detection Method	Colorimetric
Kit Components	Human Spermine oxidase microplate Human Spermine oxidase standard Human Spermine oxidase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Spermine oxidase (SMOX) ELISA Kit has high sensitivity and excellent specificity for detection of Human SMOX. No significant cross-reactivity or interference between Human SMOX and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Spermine oxidase is the polyamine oxidase. This enzyme potentially represents a new class of catabolic enzymes in the mammalian polyamine metabolic pathway capable of the efficient oxidation of polyamines. More than five transcript variants encoding four active isoenzymes have been identified for this gene, however, not all variants have been fully described. The characterized isoenzymes have distinctive biochemical characteristics and substrate specificities, suggesting the existence of additional levels of complexity in polyamine catabolism.Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal.
Notes	This Human Spermine oxidase (SMOX) ELISA Kit employs a two-site sandwich ELISA to quantitate SMOX in samples. An antibody specific for SMOX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMOX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMOX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMOX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	54498

Applications	ELISA
Applications Notes	This Human Spermine oxidase (SMOX) ELISA Kit employs a two-site sandwich ELISA to quantitate SMOX in samples. An antibody specific for SMOX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMOX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMOX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMOX bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702784	KTE60568	48 T	$339		Add to Cart
BHE13702784	KTE60568	96 T	$589		Add to Cart
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BHE13702784	KTE60568	96 Tx50	$24569		Add to Cart

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