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Home / ELISA Kit

Human Spermatogenesis-associated protein 5 (SPATA5) ELISA Kit

SKU : BHE13702762

SPATA5 encodes a member of the ATPase associated with diverse activities family, whose members are defined by a highly conserved ATPase domain. Members of this family participate in diverse cellular processes that include membrane fusion, DNA replication, microtubule severing, and protein degradation. The protein encoded by this gene has a putative mitochondrial targeting sequence and has been proposed to function in maintenance of mitochondrial function and integrity during mouse spermatogenesis. Allelic variants in SPATA5 have been associated with epilepsy, hearing loss, and mental retardation syndrome. Alternative splicing results in multiple transcript variants.

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Mfr.No.	KTE60546
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Spermatogenesis-associated protein 5 (SPATA5) ELISA Kit employs a two-site sandwich ELISA to quantitate SPATA5.
Alternative Names	SPATA5; AFG2; SPAF; ATPase family gene 2 homolog; spermatogenesis associated factor SPAF
Detection Method	Colorimetric
Kit Components	Human Spermatogenesis-associated protein 5 microplate Human Spermatogenesis-associated protein 5 standard Human Spermatogenesis-associated protein 5 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Spermatogenesis-associated protein 5 (SPATA5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPATA5. No significant cross-reactivity or interference between Human SPATA5 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SPATA5 encodes a member of the ATPase associated with diverse activities family, whose members are defined by a highly conserved ATPase domain. Members of this family participate in diverse cellular processes that include membrane fusion, DNA replication, microtubule severing, and protein degradation. The protein encoded by this gene has a putative mitochondrial targeting sequence and has been proposed to function in maintenance of mitochondrial function and integrity during mouse spermatogenesis. Allelic variants in SPATA5 have been associated with epilepsy, hearing loss, and mental retardation syndrome. Alternative splicing results in multiple transcript variants.
Notes	This Human Spermatogenesis-associated protein 5 (SPATA5) ELISA Kit employs a two-site sandwich ELISA to quantitate SPATA5 in samples. An antibody specific for SPATA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPATA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPATA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPATA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	166378

Applications	ELISA
Applications Notes	This Human Spermatogenesis-associated protein 5 (SPATA5) ELISA Kit employs a two-site sandwich ELISA to quantitate SPATA5 in samples. An antibody specific for SPATA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPATA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPATA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPATA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702762	KTE60546	48 T	$339		Add to Cart
BHE13702762	KTE60546	96 T	$589		Add to Cart
BHE13702762	KTE60546	96 Tx5	$2629		Add to Cart
BHE13702762	KTE60546	96 Tx50	$24569		Add to Cart

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