Human sperm associated antigen 9 (SPAG9) antibody ELISA Kit

Mfr.No.

CSB-E13940h

Sensitivity

20 mU/mL

Detection Range

50 mU/mL-1200 mU/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

sperm associated antigen 9 (SPAG9) antibody

Recovery

The recovery of human EPHB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.


Sample Type	?	Average % Recovery	?	Range	?
Serum (n=5)	?	99	?	95-103	?
EDTA plasma (n=4)	?	87	?	83-90	?

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human EPHB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

102

Range %

98-106

1:2

Average %

Range %

80-90

1:4

Average %

100

Range %

97-103

1:8

Average %

Range %

83-89

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected	?
4000	2.409	2.433	2.421	2.262	?
2000	1.778	1.659	1.719	1.560	?
1000	1.216	1.268	1.242	1.083	?
500	0.861	0.835	0.848	0.689	?
250	0.616	0.535	0.576	0.417	?
125	0.432	0.435	0.434	0.275	?
62.5	0.296	0.289	0.293	0.134	?
0	0.159	0.158	0.159	?	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	SPAG9 Ab

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504423	CSB-E13940h	96T	$790	Add to Cart
BHE10504423	CSB-E13940h	5x96T	$3792	Add to Cart
BHE10504423	CSB-E13940h	10x96T	$7505	Add to Cart

Human sperm associated antigen 9 (SPAG9) antibody ELISA Kit

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