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Home / ELISA Kit

Human Spectrin beta chain, brain 3 (SPTBN4) ELISA Kit

SKU : BHE13702568

Spectrin is an actin crosslinking and molecular scaffold protein that links the cell membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits.
SPTbN4 is one member of a family of beta-spectrin genes. The encoded protein localizes to the nuclear matrix, PML nuclear bodies, and cytoplasmic vesicles. A highly similar gene in the mouse is required for localization of specific membrane proteins in polarized regions of neurons. Multiple transcript variants encoding different isoforms have been found for this gene. Abundantly expressed in brain and pancreatic islets.

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Mfr.No.	KTE60351
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Spectrin beta chain, brain 3 (SPTBN4) ELISA Kit employs a two-site sandwich ELISA to quantitate SPTBN4.
Alternative Names	SPTBN4; KIAA1642; QV; SPNB4; SPTBN3; betaIV spectrin
Detection Method	Colorimetric
Kit Components	Human Spectrin beta chain, brain 3 microplate Human Spectrin beta chain, brain 3 standard Human Spectrin beta chain, brain 3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Spectrin beta chain, brain 3 (SPTBN4) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPTBN4. No significant cross-reactivity or interference between Human SPTBN4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Spectrin is an actin crosslinking and molecular scaffold protein that links the cell membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. SPTbN4 is one member of a family of beta-spectrin genes. The encoded protein localizes to the nuclear matrix, PML nuclear bodies, and cytoplasmic vesicles. A highly similar gene in the mouse is required for localization of specific membrane proteins in polarized regions of neurons. Multiple transcript variants encoding different isoforms have been found for this gene. Abundantly expressed in brain and pancreatic islets.
Notes	This Human Spectrin beta chain, brain 3 (SPTBN4) ELISA Kit employs a two-site sandwich ELISA to quantitate SPTBN4 in samples. An antibody specific for SPTBN4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPTBN4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTBN4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTBN4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	57731

Applications	ELISA
Applications Notes	This Human Spectrin beta chain, brain 3 (SPTBN4) ELISA Kit employs a two-site sandwich ELISA to quantitate SPTBN4 in samples. An antibody specific for SPTBN4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPTBN4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTBN4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTBN4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702568	KTE60351	48 T	$339		Add to Cart
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BHE13702568	KTE60351	96 Tx50	$24569		Add to Cart

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