Human SPARC-related modular calcium-binding protein 1(SMOC1) ELISA kit

Human SPARC-related modular calcium-binding protein 1(SMOC1) ELISA kit

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Mfr.No.

CSB-EL021842HU

Sensitivity

19.5 pg/mL

Detection Range

78 pg/mL-5000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

SPARC related modular calcium binding 1, secreted modular calcium-binding protein 1, SPARC related modular calcium binding 1, secreted modular calcium-binding protein 1

Recovery

The recovery of bovine IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	97	92-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-99
1:200	Average %	96
	Range %	92-101
1:400	Average %	85
	Range %	81-87
1:800	Average %	89
	Range %	83-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average
25	0.199	0.187	0.193
12.5	0.325	0.329	0.327
6.25	0.466	0.489	0.478
3.12	0.731	0.760	0.746
1.56	1.017	1.105	1.061
0.78	1.310	1.410	1.360
0.39	1.526	1.570	1.548
0	2.125	2.162	2.144

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9H4F8

Applications	ELISA
Research Area	SMOC1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504417	CSB-EL021842HU	96T	$695	Add to Cart
BHE10504417	CSB-EL021842HU	5x96T	$3336	Add to Cart
BHE10504417	CSB-EL021842HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.