Human SPAG1(Sperm-associated antigen 1) ELISA Kit

Mfr.No.

EH2525

Alternative Names

SPAG1 ELISA Kit, HSD-3.8 ELISA Kit, Infertility-related sperm protein Spag-1 ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

0.156-10ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of SPAG1 and the recovery rates were calculated by comparing the measured value to the expected amount of SPAG1 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	85-97	92
EDTA plasma(n=5)	88-105	95
heparin plasma(n=5)	89-92	90

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPAG1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	90-105%	89-103%	90-100%
EDTA plasma(n=5)	88-100%	86-100%	89-97%
heparin plasma(n=5)	86-91%	88-99%	95-97%

Sensitivity

< 0.094ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

SPAG1/HSD-3.8/Infertility-related sperm protein Spag-1

Notes

For Research Use Only

UniProt ID	Q07617
Antigen	SPAG1

Applications	ELISA
Applications Notes	SPAG1 ELISA Kit allows for the in vitro quantitative determination of SPAG1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10800728	EH2525	96T	$400		Add to Cart

Human SPAG1(Sperm-associated antigen 1) ELISA Kit

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