Human SP-D / Surfactant protein D ELISA Kit PicoKine®

Mfr.No.

EK1171

Sensitivity

<20 pg/ml

Detection Range

625 pg/ml - 40,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.049	0.188	0.306	0.514	0.979	1.383	1.708	2.056

Description

Human SP-D / Surfactant protein D ELISA Kit PicoKine® (96 Tests). Quantitate Human SFTPD in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Pulmonary surfactant-associated protein D;PSP-D;SP-D;Collectin-7;Lung surfactant protein D;SFTPD;COLEC7, PSPD, SFTP4;

Molecular Weight

37728 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A21-F375

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human SP-D

Subcellular Localization

Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1547	3580	25269	1591	3588	23717
Standard deviation	92.82	221.96	1693.02	111.37	279.86	1683.9
CV (%)	6%	6.2%	6.7%	7%	7.8%	7.1%

Protein Function

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or tuover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. .

Background

Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. Whats more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1547	1291	1390	1463	1422	94.19	6.6%
Sample 2	3580	3482	3689	3752	3625	103.31	2.8%
Sample 3	25269	29048	29153	26765	27558	1630.37	5.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6441
Gene Name	SFTPD
UniProt ID	P35247
Protein Name	Pulmonary surfactant-associated protein D
Tissue Specificity	Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes). .
Sequence	Belongs to the SFTPD family.

Applications	ELISA
Research Area	Calcium Binding Proteins,Calcium Signaling,Cancer,Cancer Metabolism,Cell Biology,Defensin,Human Protein,Immunology,Innate Immunity,Macrophage/Inflammation,Metabolism,Microbiology,Oxidative Stress,Pathways and Processes,Protein,Redox Metabolism,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000946	EK1171	96 wells/kit	$499		Add to Cart

Human SP-D / Surfactant protein D ELISA Kit PicoKine®

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