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Human Sorbitol dehydrogenase (SDH) ELISA Kit

SKU : BHE13702774

Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 (PubMed 8535074)). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.

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Mfr.No.	KTE60558
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Sorbitol dehydrogenase (SDH) ELISA Kit employs a two-site sandwich ELISA to quantitate SORD.
Alternative Names	SORD; SORD1;
Detection Method	Colorimetric
Kit Components	Human Sorbitol dehydrogenase microplate Human Sorbitol dehydrogenase standard Human Sorbitol dehydrogenase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Sorbitol dehydrogenase (SDH) ELISA Kit has high sensitivity and excellent specificity for detection of Human SORD. No significant cross-reactivity or interference between Human SORD and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 (PubMed 8535074)). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.
Notes	This Human Sorbitol dehydrogenase (SDH) ELISA Kit employs a two-site sandwich ELISA to quantitate SORD in samples. An antibody specific for SORD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySORD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SORD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SORD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6652

Applications	ELISA
Applications Notes	This Human Sorbitol dehydrogenase (SDH) ELISA Kit employs a two-site sandwich ELISA to quantitate SORD in samples. An antibody specific for SORD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySORD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SORD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SORD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702774	KTE60558	48 T	$359		Add to Cart
BHE13702774	KTE60558	96 T	$619		Add to Cart
BHE13702774	KTE60558	96 Tx5	$2809		Add to Cart
BHE13702774	KTE60558	96 Tx50	$26209		Add to Cart

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