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Home / ELISA Kit

Human Solute carrier family 22 member 12 (SLC22A12) ELISA Kit

SKU : BHE13702830

SLC22A12 is a urate transporter and urate-anion exchanger which regulates the level of urate in the blood. This protein is an integral membrane protein primarily found in kidney. Two transcript variants encoding different isoforms have been found for this gene.Detected in kidney (at protein level). Detected in fetal and adult kidney. Detected in epithelial cells of proximal tubules in renal cortex.
Numerous single nucleotide polymorphisms of this gene are significantly associated with altered (increased or decreased) reabsorption of uric acid by the kidneys. Respectively, these altered rates of reabsorption contribute to hyperuricemia and hypouricemia.

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Mfr.No.	KTE60614
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Solute carrier family 22 member 12 (SLC22A12) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC22A12.
Alternative Names	SLC22A12; OAT4L; RST; URAT1; organic anion transporter 4-like; solute carrier family 22 (organic anion/cation transporter); member 12; solute carrier family 22 member 12; urate anion exchanger 1; urate transporte
Detection Method	Colorimetric
Kit Components	Human Solute carrier family 22 member 12 microplate Human Solute carrier family 22 member 12 standard Human Solute carrier family 22 member 12 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Solute carrier family 22 member 12 (SLC22A12) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC22A12. No significant cross-reactivity or interference between Human SLC22A12 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SLC22A12 is a urate transporter and urate-anion exchanger which regulates the level of urate in the blood. This protein is an integral membrane protein primarily found in kidney. Two transcript variants encoding different isoforms have been found for this gene.Detected in kidney (at protein level). Detected in fetal and adult kidney. Detected in epithelial cells of proximal tubules in renal cortex. Numerous single nucleotide polymorphisms of this gene are significantly associated with altered (increased or decreased) reabsorption of uric acid by the kidneys. Respectively, these altered rates of reabsorption contribute to hyperuricemia and hypouricemia.
Notes	This Human Solute carrier family 22 member 12 (SLC22A12) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC22A12 in samples. An antibody specific for SLC22A12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC22A12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC22A12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC22A12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	116085

Applications	ELISA
Applications Notes	This Human Solute carrier family 22 member 12 (SLC22A12) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC22A12 in samples. An antibody specific for SLC22A12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC22A12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC22A12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC22A12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702830	KTE60614	48 T	$339		Add to Cart
BHE13702830	KTE60614	96 T	$589		Add to Cart
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BHE13702830	KTE60614	96 Tx50	$24569		Add to Cart

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