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Home / ELISA Kit

Human Solute carrier family 15 member 2 (SLC15A2) ELISA Kit

SKU : BHE13702843

This transporter, SLC15A2, belongs to the same gene family as SLC15A1, the proton-coupled peptide transporter found in the small intestine.The SLC15A2 cDNA encodes a predicted 729-amino acid protein that contains 12 transmembrane domains and a long hydrophilic segment between transmembrane domains 9 and 10.
The amino acid sequence of SLC15A2 shows 50% identity and 70% similarity with that of SLC15A1. Functional expression of the SLC15A2 cDNA in HeLa cells resulted in the induction of a proton-dependent transport system that was able to transport dipeptides, tripeptides, and beta-lactam antibiotics; free amino acids were not accepted as substrates. RT-PCR showed that SLC15A2 is expressed in kidney but not in intestine.

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Mfr.No.	KTE60627
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Solute carrier family 15 member 2 (SLC15A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC15A2.
Alternative Names	SLC15A2; FLJ33407; PEPT2; kidney H(+)/peptide cotransporter; oligopeptide transporter; kidney isoform; peptide transporter 2
Detection Method	Colorimetric
Kit Components	Human Solute carrier family 15 member 2 microplate Human Solute carrier family 15 member 2 standard Human Solute carrier family 15 member 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Solute carrier family 15 member 2 (SLC15A2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC15A2. No significant cross-reactivity or interference between Human SLC15A2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	This transporter, SLC15A2, belongs to the same gene family as SLC15A1, the proton-coupled peptide transporter found in the small intestine.The SLC15A2 cDNA encodes a predicted 729-amino acid protein that contains 12 transmembrane domains and a long hydrophilic segment between transmembrane domains 9 and 10. The amino acid sequence of SLC15A2 shows 50% identity and 70% similarity with that of SLC15A1. Functional expression of the SLC15A2 cDNA in HeLa cells resulted in the induction of a proton-dependent transport system that was able to transport dipeptides, tripeptides, and beta-lactam antibiotics; free amino acids were not accepted as substrates. RT-PCR showed that SLC15A2 is expressed in kidney but not in intestine.
Notes	This Human Solute carrier family 15 member 2 (SLC15A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC15A2 in samples. An antibody specific for SLC15A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC15A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC15A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC15A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6565

Applications	ELISA
Applications Notes	This Human Solute carrier family 15 member 2 (SLC15A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC15A2 in samples. An antibody specific for SLC15A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC15A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC15A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC15A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702843	KTE60627	48 T	$339		Add to Cart
BHE13702843	KTE60627	96 T	$589		Add to Cart
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BHE13702843	KTE60627	96 Tx50	$24569		Add to Cart

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