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Home / ELISA Kit

Human Solute carrier family 15 member 1 (SLC15A1) ELISA Kit

SKU : BHE13702844

SLC15A1 encodes an intestinal hydrogen peptide cotransporter that is a member of the solute carrier family 15. The encoded protein is localized to the brush border membrane of the intestinal epithelium and mediates the uptake of di- and tripeptides from the lumen into the enterocytes. This protein plays an important role in the uptake and digestion of dietary proteins. This protein also facilitates the absorption of numerous peptidomimetic drugs.Studies indicated that it transports dipeptides and tripeptides but not free amino acids or peptides with more than 3 amino acid residues and that its driving force for uphill transport requires proton binding and presence of an inside-negative membrane potential. A membrane protein, HTP1, which appeared to be associated with the oligopeptide transporter, had also been cloned

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Mfr.No.	KTE60628
Description	This Human Solute carrier family 15 member 1 (SLC15A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC15A1.
Alternative Names	SLC15A1; RP11-130L10.1; HPECT1; HPEPT1; PEPT1; Caco-2 oligopeptide transporter; bA551M18.1.1 (solute carrier family 15 (oligopeptide transporter) member 1); intestinal H+/peptide cotransporter; macrophage oligo
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Solute carrier family 15 member 1 microplate Human Solute carrier family 15 member 1 standard Human Solute carrier family 15 member 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Solute carrier family 15 member 1 (SLC15A1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC15A1. No significant cross-reactivity or interference between Human SLC15A1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SLC15A1 encodes an intestinal hydrogen peptide cotransporter that is a member of the solute carrier family 15. The encoded protein is localized to the brush border membrane of the intestinal epithelium and mediates the uptake of di- and tripeptides from the lumen into the enterocytes. This protein plays an important role in the uptake and digestion of dietary proteins. This protein also facilitates the absorption of numerous peptidomimetic drugs.Studies indicated that it transports dipeptides and tripeptides but not free amino acids or peptides with more than 3 amino acid residues and that its driving force for uphill transport requires proton binding and presence of an inside-negative membrane potential. A membrane protein, HTP1, which appeared to be associated with the oligopeptide transporter, had also been cloned
Notes	This Human Solute carrier family 15 member 1 (SLC15A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC15A1 in samples. An antibody specific for SLC15A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC15A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC15A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC15A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	6564
Antigen	SLC15A1
Tag	SLC15A1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Solute carrier family 15 member 1 (SLC15A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC15A1 in samples. An antibody specific for SLC15A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC15A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC15A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC15A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702844	KTE60628	48 T	$339		Add to Cart
BHE13702844	KTE60628	96 T	$589		Add to Cart
BHE13702844	KTE60628	96 Tx5	$2629		Add to Cart
BHE13702844	KTE60628	96 Tx50	$24569		Add to Cart

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