Human Soluble Urokinase Plasminogen Activating Receptor

Mfr.No.

E3759Hu

Description

This sandwich kit is for the accurate quantitative detection of human Soluble Urokinase Plasminogen Activating Receptor (also known as SUPAR) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD87;Monocyte activation antigen Mo3;PLAUR;uPAR;U-PAR;Urokinase plasminogen activator surface receptor

Reactivity

Human

Detection Range

5-1900ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

36978

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

3.82ng/L

Background

Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.

Gene ID	5329
UniProt ID	Q03405
Antigen	PLAUR

Research Area

Cancer

Protocol

These standard curves of E3759Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
960ng/L	2.11	2.252	2.208
	2.394
480ng/L	1.207	1.331	1.286
	1.455
240ng/L	0.751	0.755	0.71
	0.759
120ng/L	0.442	0.424	0.379
	0.406
60ng/L	0.273	0.264	0.22
	0.255
0ng/L	0.043	0.044	0
	0.046

Precision

We measured random samples of E3759Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	518	16.9	3.3
Intra-Assay	2	18	281.8	16.92	6
Intra-Assay	3	18	121.9	6.67	5.5

Storage	2-8°C

Reference	Soluble urokinase-type plasminogen activator receptor as a biomarker of treatment response in childhood tuberculosis Journal: Int J Mycobacteriol. Jul-Sep 2019;8(3):262-266.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105220	E3759Hu	48T	$320		Add to Cart
BHE12105220	E3759Hu	96T	$458		Add to Cart

Human Soluble Urokinase Plasminogen Activating Receptor - SUPAR ELISA Kit

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