Human soluble tumor necrosis factor-like weak inducer of apoptosis (sTWEAK)ELISA Kit

Human soluble tumor necrosis factor-like weak inducer of apoptosis (sTWEAK)ELISA Kit

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Mfr.No.

CSB-E16492h

Alternative Names

tumor necrosis factor (ligand) superfamily, member 12, APO3L, DR3LG, MGC129581, MGC20669, TWEAK, APO3/DR3 ligand, TNF-related WEAK inducer of apoptosis

Reactivity

Human

Detection Range

0.312 ng/ml-20 ng/ml

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human PLTP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	93-103
EDTA plasma (n=4)	97	90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PLTP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	94
	Range %	88-97
1:200	Average %	104
	Range %	98-107
1:400	Average %	98
	Range %	92-101
1:800	Average %	99
	Range %	93-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
150	2.667	2.547	2.607	2.510
75	1.844	1.831	1.838	1.741
37.5	1.022	1.017	1.020	0.923
18.75	0.571	0.582	0.577	0.480
9.375	0.328	0.333	0.331	0.234
4.68	0.254	0.245	0.250	0.153
2.34	0.156	0.166	0.161	0.064
0	0.096	0.098	0.097

Species

Homo sapiens

Sensitivity

0.078 ng/ml

Limit Of Detection

450 nm

UniProt ID	O43508
Antigen	tumor necrosis factor (ligand) superfamily, member 12

Applications

ELISA

Research Area

TNFSF12

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504398	CSB-E16492h	96T	$695	Add to Cart
BHE10504398	CSB-E16492h	5x96T	$3336	Add to Cart
BHE10504398	CSB-E16492h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.