Human soluble receptor activator of nuclear factor-kB ligand,sRANKL ELISA Kit

Mfr.No.

CSB-E05125h

Sensitivity

1.95 pg/mL

Detection Range

7.8 pg/mL-500 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tumor necrosis factor (ligand) superfamily, member 11, CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf, OTTHUMP00000178585, TNF-related activation-induced cytokine, osteoclast differentiation factor, osteoprotegerin ligand, receptor activator of nucle

Recovery

The recovery of mouse ChAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-100
EDTA plasma (n=4)	92	88-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ChAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	92
	Range %	88-96
1:200	Average %	87
	Range %	82-91
1:400	Average %	103
	Range %	99-108
1:800	Average %	95
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.042	2.136	2.089	1.933
500	1.407	1.481	1.444	1.288
250	0.893	0.947	0.920	0.764
125	0.610	0.658	0.634	0.478
62.5	0.475	0.463	0.469	0.313
31.2	0.321	0.305	0.313	0.157
15.6	0.254	0.249	0.252	0.096
0	0.159	0.152	0.156	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	O14788

Applications	ELISA
Research Area	TNFSF11

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504394	CSB-E05125h	96T	$790	Add to Cart
BHE10504394	CSB-E05125h	5x96T	$3792	Add to Cart
BHE10504394	CSB-E05125h	10x96T	$7505	Add to Cart

Human soluble receptor activator of nuclear factor-kB ligand,sRANKL ELISA Kit

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