Human Soluble protein-100B,S-100B ELISA Kit

Mfr.No.

CSB-E08065h

Sensitivity

19.5 pg/mL

Detection Range

78 pg/mL-5000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

S100 calcium binding protein B, NEF, S100, S100beta, OTTHUMP00000174958, S-100 calcium-binding protein, beta chain, S100 beta, S100 calcium binding protein, beta (neural), S100 calcium-binding protein, beta, S100 calcium-binding protei

Recovery

The recovery of human BIRC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	86-94
EDTA plasma (n=4)	103	98-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human BIRC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-94
1:2	Average %	92
	Range %	87-95
1:4	Average %	94
	Range %	87-98
1:8	Average %	103
	Range %	96-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2400	2.154	2.274	2.214	2.112
1200	1.268	1.318	1.293	1.191
600	0.741	0.798	0.770	0.668
300	0.443	0.460	0.452	0.350
150	0.292	0.285	0.289	0.187
75	0.167	0.174	0.171	0.069
37.5	0.132	0.139	0.136	0.034
0	0.101	0.102	0.102	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P04271

Applications	ELISA
Research Area	S100B

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504392	CSB-E08065h	96T	$835	Add to Cart
BHE10504392	CSB-E08065h	5x96T	$4008	Add to Cart
BHE10504392	CSB-E08065h	10x96T	$7932.50	Add to Cart

Human Soluble protein-100B,S-100B ELISA Kit

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