Human soluble platelet endothelial cell adhesion molecule 1,sPECAM-1 ELISA Kit

Human soluble platelet endothelial cell adhesion molecule 1,sPECAM-1 ELISA Kit

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Mfr.No.

CSB-E08133h

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

soluble platelet endothelial cell adhesion molecule 1,sPECAM-1, CD31, FLJ58394, PECAM-1, CD31 antigen, CD31/EndoCAM, PECAM-1, CD31/EndoCAM, adhesion molecule

Recovery

The recovery of human SEMA4A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	83	80-86
EDTA plasma (n=4)	87	81-90

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SEMA4A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-93
1:2	Average %	97
	Range %	93-100
1:4	Average %	91
	Range %	88-95
1:8	Average %	94
	Range %	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.604	2.635	2.620	2.478
10	1.863	1.899	1.881	1.739
5	1.021	1.036	1.029	0.887
2.5	0.564	0.532	0.548	0.406
1.25	0.315	0.335	0.325	0.183
0.625	0.259	0.271	0.265	0.123
0.312	0.206	0.199	0.203	0.061
0	0.141	0.143	0.142

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P16284

Applications	ELISA
Research Area	sPECAM1, sCD31

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504391	CSB-E08133h	96T	$595	Add to Cart
BHE10504391	CSB-E08133h	5x96T	$2856	Add to Cart
BHE10504391	CSB-E08133h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.