Human soluble OX40L (sOX40L) ELISA kit

Human soluble OX40L (sOX40L) ELISA kit

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Mfr.No.

CSB-E12752h

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tumor necrosis factor (ligand) superfamily, member 4, CD134L, CD252, GP34, OX-40L, OX4OL, TXGP1, CD134 ligand, OX40 antigen ligand, tax-transcriptionally activated glycoprotein 1 (34kD)

Recovery

The recovery of human sCD163 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	98	93-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human sCD163 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	88
	Range %	83-93
1:10	Average %	95
	Range %	89-100
1:20	Average %	97
	Range %	92-103
1:40	Average %	95
	Range %	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.452	2.552	2.502	2.368
50	2.134	2.176	2.155	2.021
25	1.539	1.497	1.518	1.384
12.5	0.728	0.739	0.734	0.600
6.25	0.412	0.424	0.418	0.284
3.12	0.229	0.239	0.234	0.100
1.56	0.194	0.201	0.198	0.064
0	0.132	0.135	0.134	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P23510

Applications	ELISA
Research Area	TNFSF4

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504390	CSB-E12752h	96T	$790	Add to Cart
BHE10504390	CSB-E12752h	5x96T	$3792	Add to Cart
BHE10504390	CSB-E12752h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.