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Home / ELISA Kit

Human Soluble myosin heavy chain 1 (sMHC-1) ELISA Kit

SKU : BHE13703662

Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.

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Mfr.No.	KTE61447
Description	This Human Soluble myosin heavy chain 1 (sMHC-1) ELISA Kit employs a two-site sandwich ELISA to quantitate MYH1.
Alternative Names	MYH1; MGC133384; MYHSA1; MYHa; MyHC-2X/D; myosin heavy chainx/d; myosin; heavy polypeptide 1; skeletal muscle; adult
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Soluble myosin heavy chain 1 microplate Human Soluble myosin heavy chain 1 standard Human Soluble myosin heavy chain 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Soluble myosin heavy chain 1 (sMHC-1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYH1. No significant cross-reactivity or interference between Human MYH1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.
Notes	This Human Soluble myosin heavy chain 1 (sMHC-1) ELISA Kit employs a two-site sandwich ELISA to quantitate MYH1 in samples. An antibody specific for MYH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	4619
Antigen	MYH1
Tag	MYH1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Soluble myosin heavy chain 1 (sMHC-1) ELISA Kit employs a two-site sandwich ELISA to quantitate MYH1 in samples. An antibody specific for MYH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703662	KTE61447	48 T	$339		Add to Cart
BHE13703662	KTE61447	96 T	$589		Add to Cart
BHE13703662	KTE61447	96 Tx5	$2629		Add to Cart
BHE13703662	KTE61447	96 Tx50	$24569		Add to Cart

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