Human Soluble Interleukin-7 Receptor,sIL-7R ELISA Kit

Human Soluble Interleukin-7 Receptor,sIL-7R ELISA Kit

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Mfr.No.

CSB-E14020h

Alternative Names

interleukin 7 receptor, CD127, CDW127, IL-7R-alpha, IL7RA, ILRA, CD127 antigen, interleukin 7 receptor alpha chain, interleukin 7 receptor isoform H5-6

Reactivity

Human

Detection Range

0.78 ng/mL-50 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse CTSS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-98
EDTA plasma (n=4)	87	83-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTSS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-92
1:2	Average %	95
	Range %	91-105
1:4	Average %	96
	Range %	90-101
1:8	Average %	93
	Range %	85-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.884	2.952	2.918	2.750
1000	2.345	2.452	2.399	2.231
500	1.652	1.549	1.601	1.433
250	0.729	0.739	0.734	0.566
125	0.457	0.452	0.455	0.287
62.5	0.285	0.297	0.291	0.123
31.25	0.216	0.219	0.218	0.050
0	0.168	0.167	0.168	?

Species

Homo sapiens

Sensitivity

0.195 ng/mL

Limit Of Detection

450 nm

UniProt ID	P16871
Antigen	Interleukin 7 Receptor

Applications

ELISA

Research Area

IL7R

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504385	CSB-E14020h	96T	$790	Add to Cart
BHE10504385	CSB-E14020h	5x96T	$3792	Add to Cart
BHE10504385	CSB-E14020h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.